During the process of gene amplification using PCR, if very high temperature is not maintained in the beginning, then which of the following steps of PCR

During the process of gene amplification using PCR, if very high temperature is not maintained in the beginning, then which of the following steps of PCR will be affected first ?

(1) Ligation (2) Annealing (3) Extension (4) Denaturation

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During the process of gene amplification using PCR, if very high temperature is not maintained in the beginning, then which of the following steps of PCR

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