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Topic: DNA replication and central dogma· Genetics and Evolution

Which of the following statement is correct regarding the process of replication in E.coli?

1. The DNA dependent DNA polymerase catalyses polymerization in \(5' \rightarrow 3'\) direction.

2. The DNA dependent DNA polymerase catalyses polymerization in one direction that is \(3' \rightarrow 5'\).

3. The DNA dependent RNA polymerase catalyses polymerization in one direction, that is \(5' \rightarrow 3'\).

4. The DNA dependent DNA polymerase catalyses polymerization in \(5' \rightarrow 3'\) as well as \(3' \rightarrow 5'\) direction.

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Answer: 1. The DNA dependent DNA polymerase catalyses polymerization in \(5' \rightarrow 3'\) direction.

Key idea: DNA polymerases add nucleotides only in the 5' to 3' direction.

The DNA-dependent DNA polymerase extends a chain only at its 3'-OH end, so it polymerises 5'\(\to\)3'. That is why the lagging strand is made in fragments. Options 2 and 4 name a wrong direction, and option 3 names RNA polymerase, which is about transcription.

Doubtly AI · 30 Oct 2026 AI-generated · not yet verified Report
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