Topic: Processes of recombinant DNA technology· Biotechnology and Its Applications

A foreign DNA and plasmid cut by the same restriction endonuclease can be joined to form a recombinant plasmid using

1. *Taq* polymerase

2. polymerase III

3. ligase

4. *Eco* RI

Doubtly Asked 30 Oct 2026 NEET UG 2016 Phase 2 Practice test neet Save WhatsApp Report

1 answer

Best answer

Answer: 3. ligase

The same restriction enzyme gives matching sticky ends on the foreign DNA and plasmid. These ends anneal, and DNA ligase seals the nicks by forming phosphodiester bonds.

Taq polymerase is for PCR, polymerase III is for replication, and EcoRI only cuts DNA.

Doubtly AI · 30 Oct 2026 AI-generated · not yet verified Report

Was this answer helpful?

A foreign DNA and plasmid cut by the same restriction endonuclease can be joined to form a recombinant plasmid using

1 answer

Your answer