Processes of recombinant DNA technology
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See all →- Given below are two statements :Statement I : The DNA fragments extracted from gel electrophoresis can be used in construction of recombinant DNA.Statement II : Smaller size DNA fragments are observed near anode while larger fragments are found near… Answer: 1. Both statement I and statement II are correct Key idea: Gel electrophoresis separates DNA by size, and the separated bands can be... 1
- Identify the part of a bio-reactor which is used as a foam braker from the given figure. Answer: 4. C Key idea: In a stirred-tank bioreactor the foam breaker is a blade on the shaft near the top, in the headspace above the cultur... 1
- Polymerase chain reaction (PCR) amplifies DNA following the equation. Answer: 2. 2ⁿ Key idea: In each PCR cycle every DNA molecule is copied once, so the number of copies doubles. Starting from one DNA molecule... 1
- Which of the following statements is incorrect? Answer: 4. Bio-reactors are used to produce small scale bacterial cultures. Bioreactors are large vessels (hundreds to thousands of litres)... 1
- What is the fate of a piece of DNA carrying only gene of interest which is transferred into an alien organism?A. The piece of DNA would be able to multiply itself independently in the progeny cells of the organism.B. It may get integrated into the ge… Answer: 4. B and C only Key idea: a piece of alien DNA without an origin of replication cannot multiply on its own. It either integrates int... 1
- In gene gun method used to introduce alien DNA into host cells, microparticles of ________ metal are used. Answer: 4. Tungsten or gold Key idea: In the biolistic (gene gun) method, DNA is coated on microscopic particles of tungsten or gold, which... 1