Biotechnology and Its Applications
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See all →- Why can't insulin be given orally to diabetic patients? Answer: 2. It will be digested in Gastro-Intestinal (GI) tract Key idea: Insulin is a protein (peptide hormone). If taken orally, it is dige... 1
- Which of the following enzyme(s) are NOT essential for gene cloning?A. Restriction enzymesB. DNA ligaseC. DNA mutaseD. DNA recombinaseE. DNA polymeraseChoose the correct answer from the options given below : Answer: 1. C and D only Key idea: Gene cloning needs enzymes to cut (restriction enzymes), join (DNA ligase), and make copies (DNA polymeras... 1
- The blue and white selectable markers have been developed which differentiate recombinant colonies from non-recombinant colonies on the basis of their ability to produce colour in presence of a chromogenic substrate.Given below are two statements abo… Answer: 4. Statement I is incorrect but Statement II is correct Key idea: Insertional inactivation of the lacZ gene (β-galactosidase). In a... 1
- Silencing of specific mRNA is possible via RNAi because of - Answer: 1. Complementary dsRNA Key idea: RNA interference (RNAi) works through double-stranded RNA. A dsRNA complementary to a specific mRNA... 1
- Given below are two statements :Statement I : The DNA fragments extracted from gel electrophoresis can be used in construction of recombinant DNA.Statement II : Smaller size DNA fragments are observed near anode while larger fragments are found near… Answer: 1. Both statement I and statement II are correct Key idea: Gel electrophoresis separates DNA by size, and the separated bands can be... 1
- Which of the following genetically engineered organisms was used by Eli Lilly to prepare human insulin? Answer: 1. Bacterium Key idea: Eli Lilly produced human insulin in 1983 using the bacterium E. coli. They made the A and B chains separately... 1