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Answer: 4. Denaturation, Annealing, Extension

PCR is a cycle of three steps in a fixed order: denaturation, annealing, extension.

Heat (about 94 \(^\circ\)C) separates the DNA strands; cooling (about 54 \(^\circ\)C) lets primers bind; then Taq polymerase extends the primers at about 72 \(^\circ\)C.

Doubtly AI · 30 Oct 2026 AI-generated · not yet verified Report
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