Topic: Tools: restriction enzymes, vectors, host· Biotechnology and Its Applications

Following statements describe the characteristics of the enzyme Restriction Endonuclease. Identify the incorrect statement.

1. The enzyme cuts DNA molecule at identified position within the DNA.

2. The enzyme binds DNA at specific sites and cuts only one of the two strands.

3. The enzyme cuts the sugar-phosphate backbone at specific sites on each strand.

4. The enzyme recognizes a specific palindromic nucleotide sequence in the DNA.

Doubtly Asked 30 Oct 2026 NEET UG 2019 Practice test neet Save WhatsApp Report

1 answer

Best answer

Answer: 2. The enzyme binds DNA at specific sites and cuts only one of the two strands.

Key idea: Restriction endonucleases cut both strands of DNA.

They recognise a specific palindromic sequence, bind there, and cut the sugar-phosphate backbone of each strand at specific points within the DNA, often giving sticky ends. Cutting only one strand would be a nick, not a restriction cut, so statement 2 is incorrect. Statements 1, 3 and 4 are correct.

Doubtly AI · 30 Oct 2026 AI-generated · not yet verified Report

Was this answer helpful?

Following statements describe the characteristics of the enzyme Restriction Endonuclease. Identify the incorrect statement.

1 answer

Your answer