Tools: restriction enzymes, vectors, host
Top questions
See all →- Which of the following enzyme(s) are NOT essential for gene cloning?A. Restriction enzymesB. DNA ligaseC. DNA mutaseD. DNA recombinaseE. DNA polymeraseChoose the correct answer from the options given below : Answer: 1. C and D only Key idea: Gene cloning needs enzymes to cut (restriction enzymes), join (DNA ligase), and make copies (DNA polymeras... 1
- The blue and white selectable markers have been developed which differentiate recombinant colonies from non-recombinant colonies on the basis of their ability to produce colour in presence of a chromogenic substrate.Given below are two statements abo… Answer: 4. Statement I is incorrect but Statement II is correct Key idea: Insertional inactivation of the lacZ gene (β-galactosidase). In a... 1
- In the above represented plasmid an alien piece of DNA is inserted at EcoRI site. Which of the following strategies will be chosen to select the recombinant colonies? Answer: 3. White color colonies will be selected. Key idea: The figure shows the EcoRI site inside the gene for β -galactosidase. Inserting... 1
- The "Ti plasmid" of Agrobacterium tumefaciens stands for Answer: 4. Tumor inducing plasmid The Ti plasmid of Agrobacterium tumefaciens is the tumour-inducing plasmid. Part of it (T-DNA) enters plan... 1
- The following diagram showing restriction sites in E.coli cloning vector pBR322. Find the role of 'X' and 'Y' genes : Answer: 3. The gene 'X' is responsible for controlling the copy number of the linked DNA and 'Y' for protein involved in the replication of... 1
- Hind II always cuts DNA molecules at a particular point called recognition sequence and it consists of: Answer: 3. 6 bp Key idea: restriction enzymes such as Hind II recognise a specific palindromic sequence of 6 base pairs. Hind II recognises... 1