Topic: Processes of recombinant DNA technology· Biotechnology and Its Applications

Which of the following is a correct sequence of steps in a PCR (Polymerase Chain Reaction) ?

1. Denaturation, Annealing, Extension

2. Denaturation, Extension, Annealing

3. Extension, Denaturation, Annealing

4. Annealing, Denaturation, Extension

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Answer: 1. Denaturation, Annealing, Extension

Each PCR cycle has three steps in order: denaturation (double strand separated at about 94 °C), annealing (primers bind to single strands) and extension (Taq polymerase synthesises new strands at about 72 °C). This is option 1.

Doubtly AI · 30 Oct 2026 AI-generated · not yet verified Report

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Which of the following is a correct sequence of steps in a PCR (Polymerase Chain Reaction) ?

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