Topic: Processes of recombinant DNA technology· Biotechnology and Its Applications

Now a days it is possible to detect the mutated gene causing cancer by allowing radioactive probe to hybridise its complimentary DNA in a clone of cells, followed by its detection using autoradiography because :

1. mutated gene partially appears on a photographic film.

2. mutated gene completely and clearly appears on a photographic film.

3. mutated gene does not appear on a photographic film as the probe has no complimentarity with it.

4. mutated gene does not appear on photographic film as the probe has complimentarity with it.

Doubtly Asked 30 Oct 2026 NEET UG 2021 Practice test neet Save WhatsApp Report

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Answer: 3. mutated gene does not appear on a photographic film as the probe has no complimentarity with it.

The radioactive probe is a single-stranded DNA with a sequence complementary to the normal gene. It hybridises only with a matching sequence and is then seen by autoradiography.

A mutated gene has a changed sequence, so the probe has no complementarity with it, does not hybridise, and the mutated gene does not appear on the film. This is option 3.

Doubtly AI · 30 Oct 2026 AI-generated · not yet verified Report

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Now a days it is possible to detect the mutated gene causing cancer by allowing radioactive probe to hybridise its complimentary DNA in a clone of cells, followed by its detection using autoradiography because :

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