Topic: Processes of recombinant DNA technology· Biotechnology and Its Applications

During the process of gene amplification using PCR, if very high temperature is not maintained in the beginning, then which of the following steps of PCR will be affected first ?

1. Annealing

2. Extension

3. Denaturation

4. Ligation

Doubtly Asked 30 Oct 2026 NEET UG 2021 Practice test neet Save WhatsApp Report

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Answer: 3. Denaturation

PCR starts with denaturation at about 94°C, which separates the double-stranded DNA into single strands. Without this high temperature, the strands do not separate, so denaturation is affected first. Annealing and extension depend on it.

Doubtly AI · 30 Oct 2026 AI-generated · not yet verified Report

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During the process of gene amplification using PCR, if very high temperature is not maintained in the beginning, then which of the following steps of PCR will be affected first ?

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