Topic: Processes of recombinant DNA technology· Biotechnology and Its Applications

Which one of the following statement is not true regarding gel electrophoresis technique ?

1. Bright orange coloured bands of DNA can be observed in the gel when exposed to UV light.

2. The process of extraction of separated DNA strands from gel is called elution.

3. The separated DNA fragments are stained by using ethidium bromide.

4. The presence of chromogenic substrate gives blue coloured DNA bands on the gel.

Doubtly Asked 30 Oct 2026 NEET UG 2022 Practice test neet Save WhatsApp Report

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Answer: 4. The presence of chromogenic substrate gives blue coloured DNA bands on the gel.

In gel electrophoresis, DNA fragments are stained with ethidium bromide and seen as bright orange bands under UV light, then cut out and eluted.

Statements 1, 2 and 3 are true. Chromogenic substrates are used to select recombinants in blue-white screening of colonies, not to colour DNA bands on a gel. So option 4 is not true.

Doubtly AI · 30 Oct 2026 AI-generated · not yet verified Report

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Which one of the following statement is not true regarding gel electrophoresis technique ?

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