Topic: Tools: restriction enzymes, vectors, host· Biotechnology and Its Applications

In the following palindromic base sequences of DNA, which one can be cut easily by particular restriction enzyme ?

1. 5'GTATTC3'; 3'CATAAG5'

2. 5'GATACT3'; 3'CTATGA5'

3. 5'GAATTC3'; 3'CTTAAG5'

4. 5'CTCAGT3'; 3'GAGTCA5'

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Answer: 3. 5'GAATTC3'; 3'CTTAAG5'

A palindromic site reads the same 5' to 3' on both strands. 5'GAATTC3' with complementary 3'CTTAAG5' is a palindrome, the EcoRI site, so this is cut easily. The other sequences are not palindromic.

Doubtly AI · 30 Oct 2026 AI-generated · not yet verified Report

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In the following palindromic base sequences of DNA, which one can be cut easily by particular restriction enzyme ?

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