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Topic: Tools: restriction enzymes, vectors, host· Biotechnology and Its Applications

Given below are two statements :Statement I :Restriction endonucleases recognise specific sequence to cut DNA known as palindromic nucleotide sequence.Statement II :Restriction endonucleases cut the DNA strand a little away from the centre of the pal…

Given below are two statements :

Statement I :

Restriction endonucleases recognise specific sequence to cut DNA known as palindromic nucleotide sequence.

Statement II :

Restriction endonucleases cut the DNA strand a little away from the centre of the palindromic site.

In the light of the above statements, choose the most appropriate answer from the options given below :

1. Statement I is incorrect but Statement II is correct

2. Both Statement I and Statement II are correct

3. Both Statement I and Statement II are incorrect

4. Statement I is correct but Statement II is incorrect

1 answer

Best answer

Answer: 2. Both Statement I and Statement II are correct

Key idea: Restriction enzymes recognise palindromic sequences and cut each strand slightly off the centre.

  • Statement I: a palindromic sequence reads the same on both strands in the 5'→3' direction, for example GAATTC. Restriction endonucleases recognise such sites. Correct.
  • Statement II: the enzyme cuts the two strands a little away from the centre of the palindrome, which leaves sticky (staggered) ends. Correct.

Both are correct: option 2.

Doubtly AI · 30 Oct 2026 AI-generated · not yet verified Report
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