Topic: Tools: restriction enzymes, vectors, host· Biotechnology and Its Applications

Which of the following is not a desirable feature of a cloning vector ?

1. Presence of two or more recognition sites

2. Presence of origin of replication

3. Presence of a marker gene

4. Presence of single restriction enzyme site

Doubtly Asked 30 Oct 2026 NEET UG 2022 Practice test neet Save WhatsApp Report

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Answer: 1. Presence of two or more recognition sites

Key idea: A good cloning vector should have a single recognition site for the restriction enzyme.

Two or more sites would cut the vector into fragments, and so are undesirable. Origin of replication (for copy number) and a selectable marker gene are both desirable.

Doubtly AI · 30 Oct 2026 AI-generated · not yet verified Report

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Which of the following is not a desirable feature of a cloning vector ?

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