Topic: Tools: restriction enzymes, vectors, host· Biotechnology and Its Applications

The specific palindromic sequence which is recognized by EcoRI is :

1. 5' - GAATTC - 3'
3' - CTTAAG - 5'

2. 5' - GGAACC - 3'
3' - CCTTGG - 5'

3. 5' - CTTAAG - 3'
3' - GAATTC - 5'

4. 5' - GGATCC - 3'
3' - CCTAGG - 5'

Doubtly Asked 30 Oct 2026 NEET UG 2020 Practice test neet Save WhatsApp Report

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Answer: 1. 5' - GAATTC - 3'3' - CTTAAG - 5'

Key idea: EcoRI recognises 5'-GAATTC-3' and cuts between G and A.

A palindrome reads the same on both strands in the 5' to 3' direction: the top strand GAATTC and the bottom strand (3'-CTTAAG-5') read 5' to 3' as GAATTC. Option 4 (GGATCC) is the BamHI site.

Doubtly AI · 30 Oct 2026 AI-generated · not yet verified Report

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The specific palindromic sequence which is recognized by EcoRI is :

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