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Processes of recombinant DNA technology

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Every question from past board papers on Processes of recombinant DNA technology, newest paper first. Open any question for its full worked answer.

NEET UG 2025 question paper (official, booklet 45)

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  1. Polymerase chain reaction (PCR) amplifies DNA following the equation.

    4 marks · See worked answer →

  2. Identify the part of a bio-reactor which is used as a foam braker from the given figure.

    Stirred-tank bioreactor with parts labelled A, B, C, D

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  3. Given below are two statements :

    Statement I : The DNA fragments extracted from gel electrophoresis can be used in construction of recombinant DNA.

    Statement II : Smaller size DNA fragments are observed near anode while larger fragments are found near the wells in an agarose gel.

    In the light of the above statements, choose the most appropriate answer from the options given below :

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NEET UG 2024 question paper (official, booklet S6)

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  1. What is the fate of a piece of DNA carrying only gene of interest which is transferred into an alien organism?

    A. The piece of DNA would be able to multiply itself independently in the progeny cells of the organism.

    B. It may get integrated into the genome of the recipient.

    C. It may multiply and be inherited along with the host DNA.

    D. The alien piece of DNA is not an integral part of chromosome.

    E. It shows ability to replicate.

    Choose the correct answer from the options given below:

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  2. Which of the following statements is incorrect?

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NEET UG 2023 question paper (official, booklet G6)

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  1. Upon exposure to UV radiation, DNA stained with ethidium bromide will show

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  2. During the purification process for recombinant DNA technology, addition of chilled ethanol precipitates out

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  3. In gene gun method used to introduce alien DNA into host cells, microparticles of ________ metal are used.

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  4. Main steps in the formation of Recombinant DNA are given below. Arrange these steps in a correct sequence.

    A. Insertion of recombinant DNA into the host cell.

    B. Cutting of DNA at specific location by restriction enzyme.

    C. Isolation of desired DNA fragment.

    D. Amplification of gene of interest using PCR.

    Choose the correct answer from the options given below :

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NEET UG 2022 question paper (official, booklet R6)

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  1. Given below are two statements : one is labelled as Assertion (A) and the other is labelled as Reason (R).

    Assertion (A) :

    Polymerase chain reaction is used in DNA amplification

    Reason (R) :

    The ampicillin resistant gene is used as a selectable marker to check transformation

    In the light of the above statements, choose the correct answer from the options given below :

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  2. Which one of the following statement is not true regarding gel electrophoresis technique ?

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NEET UG 2021 question paper (official, booklet M4)

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  1. Which of the following is not an application of PCR (Polymerase Chain Reaction) ?

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  2. During the purification process for recombinant DNA technology, addition of chilled ethanol precipitates out :

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  3. Which of the following is a correct sequence of steps in a PCR (Polymerase Chain Reaction) ?

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  4. Now a days it is possible to detect the mutated gene causing cancer by allowing radioactive probe to hybridise its complimentary DNA in a clone of cells, followed by its detection using autoradiography because :

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  5. For effective treatment of the disease, early diagnosis and understanding its pathophysiology is very important. Which of the following molecular diagnostic techniques is very useful for early detection ?

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  6. During the process of gene amplification using PCR, if very high temperature is not maintained in the beginning, then which of the following steps of PCR will be affected first ?

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NEET UG 2020 question paper (official, booklet E1)

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  1. In gel electrophoresis, separated DNA fragments can be visualized with the help of :

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NEET UG 2019 question paper (official, booklet P1)

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  1. Which one of the following equipments is essentially required for growing microbes on a large scale, for industrial production of enzymes ?

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  2. DNA precipitation out of a mixture of biomolecules can be achieved by treatment with :

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NEET UG 2018 question paper (official, booklet EE)

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  1. The correct order of steps in Polymerase Chain Reaction (PCR) is

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NEET UG 2017 question paper (official, booklet B)

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  1. DNA fragments are :

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  2. The process of separation and purification of expressed protein before marketing is called :

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  3. The DNA fragments separated on an agarose gel can be visualised after staining with :

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  4. What is the criterion for DNA fragments movement on agarose gel during gel electrophoresis ?

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NEET UG 2016 Phase 1 question paper (official, booklet A)

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  1. The taq polymerase enzyme is obtained from :

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NEET UG 2016 Phase 2 question paper (official, booklet ZZ)

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  1. Stirred-tank bioreactors have been designed for

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  2. A foreign DNA and plasmid cut by the same restriction endonuclease can be joined to form a recombinant plasmid using

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  3. Which of the following is not a component of downstream processing?

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