Polymerase chain reaction (PCR) amplifies DNA following the equation.
Biotechnology and Its Applications
Every question from past board papers on Biotechnology and Its Applications, newest paper first. Open any question for its full worked answer.
NEET UG 2025 question paper (official, booklet 45)
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In the above represented plasmid an alien piece of DNA is inserted at EcoRI site. Which of the following strategies will be chosen to select the recombinant colonies?
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Which of the following genetically engineered organisms was used by Eli Lilly to prepare human insulin?
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Identify the part of a bio-reactor which is used as a foam braker from the given figure.
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Given below are two statements :
Statement I : The DNA fragments extracted from gel electrophoresis can be used in construction of recombinant DNA.
Statement II : Smaller size DNA fragments are observed near anode while larger fragments are found near the wells in an agarose gel.
In the light of the above statements, choose the most appropriate answer from the options given below :
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Silencing of specific mRNA is possible via RNAi because of -
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Why can't insulin be given orally to diabetic patients?
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Which of the following enzyme(s) are NOT essential for gene cloning?
A. Restriction enzymes
B. DNA ligase
C. DNA mutase
D. DNA recombinase
E. DNA polymerase
Choose the correct answer from the options given below :
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The blue and white selectable markers have been developed which differentiate recombinant colonies from non-recombinant colonies on the basis of their ability to produce colour in presence of a chromogenic substrate.
Given below are two statements about this method:
Statement I : The blue coloured colonies have DNA insert in the plasmid and they are identified as recombinant colonies.
Statement II : The colonies without blue colour have DNA insert in the plasmid and are identified as recombinant colonies.
In the light of the above statements, choose the most appropriate answer from the options given below :
NEET UG 2024 question paper (official, booklet S6)
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What is the fate of a piece of DNA carrying only gene of interest which is transferred into an alien organism?
A. The piece of DNA would be able to multiply itself independently in the progeny cells of the organism.
B. It may get integrated into the genome of the recipient.
C. It may multiply and be inherited along with the host DNA.
D. The alien piece of DNA is not an integral part of chromosome.
E. It shows ability to replicate.
Choose the correct answer from the options given below:
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Given below are two statements:
Statement I : Bt toxins are insect group specific and coded by a gene cry IAc.
Statement II : Bt toxin exists as inactive protoxin in B. thuringiensis. However, after ingestion by the insect the inactive protoxin gets converted into active form due to acidic pH of the insect gut.
In the light of the above statements, choose the correct answer from the options given below:
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Hind II always cuts DNA molecules at a particular point called recognition sequence and it consists of:
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Match List I with List II :
List I:
A. α-1 antitrypsin
B. Cry IAb
C. Cry IAc
D. Enzyme replacement therapy
List II:
I. Cotton bollworm
II. ADA deficiency
III. Emphysema
IV. Corn borer
Choose the correct answer from the options given below :
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The "Ti plasmid" of Agrobacterium tumefaciens stands for
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The following diagram showing restriction sites in E.coli cloning vector pBR322. Find the role of 'X' and 'Y' genes :
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Which of the following statements is incorrect?
NEET UG 2023 question paper (official, booklet G6)
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Upon exposure to UV radiation, DNA stained with ethidium bromide will show
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During the purification process for recombinant DNA technology, addition of chilled ethanol precipitates out
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In gene gun method used to introduce alien DNA into host cells, microparticles of ________ metal are used.
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Main steps in the formation of Recombinant DNA are given below. Arrange these steps in a correct sequence.
A. Insertion of recombinant DNA into the host cell.
B. Cutting of DNA at specific location by restriction enzyme.
C. Isolation of desired DNA fragment.
D. Amplification of gene of interest using PCR.
Choose the correct answer from the options given below :
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Which one of the following techniques does not serve the purpose of early diagnosis of a disease for its early treatment?
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Which of the following is not a cloning vector?
NEET UG 2022 question paper (official, booklet R6)
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Given below are two statements : one is labelled as Assertion (A) and the other is labelled as Reason (R).
Assertion (A) :
Polymerase chain reaction is used in DNA amplification
Reason (R) :
The ampicillin resistant gene is used as a selectable marker to check transformation
In the light of the above statements, choose the correct answer from the options given below :
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Which one of the following statement is not true regarding gel electrophoresis technique ?
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In the following palindromic base sequences of DNA, which one can be cut easily by particular restriction enzyme ?
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Transposons can be used during which one of the following ?
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Given below are two statements :
Statement I :
Restriction endonucleases recognise specific sequence to cut DNA known as palindromic nucleotide sequence.
Statement II :
Restriction endonucleases cut the DNA strand a little away from the centre of the palindromic site.
In the light of the above statements, choose the most appropriate answer from the options given below :
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In gene therapy of Adenosine Deaminase (ADA) deficiency, the patient requires periodic infusion of genetically engineered lymphocytes because :
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Statements related to human Insulin are given below. Which statement(s) is/are correct about genetically engineered Insulin ?
(a) Pro-hormone insulin contain extra stretch of C-peptide
(b) A-peptide and B-peptide chains of insulin were produced separately in E.coli, extracted and combined by creating disulphide bond between them.
(c) Insulin used for treating Diabetes was extracted from Cattles and Pigs.
(d) Pro-hormone Insulin needs to be processed for converting into a mature and functional hormone.
(e) Some patients develop allergic reactions to the foreign insulin.
Choose the most appropriate answer from the options given below :
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Which of the following is not a desirable feature of a cloning vector ?
NEET UG 2021 question paper (official, booklet M4)
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Which of the following is not an application of PCR (Polymerase Chain Reaction) ?
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During the purification process for recombinant DNA technology, addition of chilled ethanol precipitates out :
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When gene targetting involving gene amplification is attempted in an individual’s tissue to treat disease, it is known as :
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Which of the following is a correct sequence of steps in a PCR (Polymerase Chain Reaction) ?
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Plasmid pBR322 has PstI restriction enzyme site within gene ampR that confers ampicillin resistance. If this enzyme is used for inserting a gene for β-galactoside production and the recombinant plasmid is inserted in an E.coli strain
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Now a days it is possible to detect the mutated gene causing cancer by allowing radioactive probe to hybridise its complimentary DNA in a clone of cells, followed by its detection using autoradiography because :
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A specific recognition sequence identified by endonucleases to make cuts at specific positions within the DNA is :
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With regard to insulin choose correct options.
(a) C-peptide is not present in mature insulin.
(b) The insulin produced by rDNA technology has C-peptide.
(c) The pro-insulin has C-peptide.
(d) A-peptide and B-peptide of insulin are interconnected by disulphide bridges.
Choose the correct answer from the options given below.
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For effective treatment of the disease, early diagnosis and understanding its pathophysiology is very important. Which of the following molecular diagnostic techniques is very useful for early detection ?
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During the process of gene amplification using PCR, if very high temperature is not maintained in the beginning, then which of the following steps of PCR will be affected first ?
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The Adenosine deaminase deficiency results into :
NEET UG 2020 question paper (official, booklet E1)
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Choose the correct pair from the following :
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In gel electrophoresis, separated DNA fragments can be visualized with the help of :
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The specific palindromic sequence which is recognized by EcoRI is :
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Identify the wrong statement with regard to Restriction Enzymes.
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Match the organism with its use in biotechnology.
(a) Bacillus thuringiensis (i) Cloning vector
(b) Thermus aquaticus (ii) Construction of first rDNA molecule
(c) Agrobacterium tumefaciens (iii) DNA polymerase
(d) Salmonella typhimurium (iv) Cry proteins
Select the correct option from the following :
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Which of the following statements is not correct ?
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Bt cotton variety that was developed by the introduction of toxin gene of Bacillus thuringiensis (Bt) is resistant to :
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Match the following columns and select the correct option.
Column - I | Column - II
(a) Bt cotton | (i) Gene therapy
(b) Adenosine deaminase deficiency | (ii) Cellular defence
(c) RNAi | (iii) Detection of HIV infection
(d) PCR | (iv) Bacillus thuringiensis
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The sequence that controls the copy number of the linked DNA in the vector, is termed :
NEET UG 2019 question paper (official, booklet P1)
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Which one of the following equipments is essentially required for growing microbes on a large scale, for industrial production of enzymes ?
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DNA precipitation out of a mixture of biomolecules can be achieved by treatment with :
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Following statements describe the characteristics of the enzyme Restriction Endonuclease. Identify the incorrect statement.
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Which of the following is true for Golden rice ?
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What triggers activation of protoxin to active Bt toxin of \(\textit{Bacillus thuringiensis}\) in boll worm ?
NEET UG 2018 question paper (official, booklet EE)
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Which of the following is commonly used as a vector for introducing a DNA fragment in human lymphocytes ?
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The correct order of steps in Polymerase Chain Reaction (PCR) is
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In India, the organisation responsible for assessing the safety of introducing genetically modified organisms for public use is
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Use of bioresources by multinational companies and organisations without authorisation from the concerned country and its people is called
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A ‘new’ variety of rice was patented by a foreign company, though such varieties have been present in India for a long time. This is related to
NEET UG 2017 question paper (official, booklet B)
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DNA fragments are :
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The process of separation and purification of expressed protein before marketing is called :
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The DNA fragments separated on an agarose gel can be visualised after staining with :
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What is the criterion for DNA fragments movement on agarose gel during gel electrophoresis ?
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A gene whose expression helps to identify transformed cell is known as :
NEET UG 2016 Phase 1 question paper (official, booklet A)
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Which of the following is not a feature of the plasmids ?
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The taq polymerase enzyme is obtained from :
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Which part of the tobacco plant is infected by *Meloidogyne incognita* ?
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Which of the following is a restriction endonuclease ?
NEET UG 2016 Phase 2 question paper (official, booklet ZZ)
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Stirred-tank bioreactors have been designed for
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A foreign DNA and plasmid cut by the same restriction endonuclease can be joined to form a recombinant plasmid using
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Which of the following is not a component of downstream processing?
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Which of the following restriction enzymes produces blunt ends?
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Which kind of therapy was given in 1990 to a four-year-old girl with adenosine deaminase (ADA) deficiency?