Applications in health: human insulin, vaccines, gene therapy
- Why can't insulin be given orally to diabetic patients? Answer: 2. It will be digested in Gastro-Intestinal (GI) tract Key idea: Insulin is a protein (peptide hormone). If taken orally, it is dige... 1
- Which of the following genetically engineered organisms was used by Eli Lilly to prepare human insulin? Answer: 1. Bacterium Key idea: Eli Lilly produced human insulin in 1983 using the bacterium E. coli. They made the A and B chains separately... 1
- Match List I with List II :List I:A. α-1 antitrypsinB. Cry IAbC. Cry IAcD. Enzyme replacement therapyList II:I. Cotton bollwormII. ADA deficiencyIII. EmphysemaIV. Corn borerChoose the correct answer from the options given below : Answer: 4. A-III, B-IV, C-I, D-II Key idea: link each biotech product or therapy with its use. α-1 antitrypsin - treats emphysema (III). Cry... 1
- Which one of the following techniques does not serve the purpose of early diagnosis of a disease for its early treatment? Answer: 3. Serum and Urine analysis Key idea: Early diagnosis needs methods that detect a pathogen or a gene before symptoms appear. PCR amp... 1
- Statements related to human Insulin are given below. Which statement(s) is/are correct about genetically engineered Insulin ?(a) Pro-hormone insulin contain extra stretch of C-peptide(b) A-peptide and B-peptide chains of insulin were produced separat… Answer: 3. (b) only Key idea: The question is about genetically engineered insulin. (b) Correct: A and B chains were made separately in E. c... 1
- In gene therapy of Adenosine Deaminase (ADA) deficiency, the patient requires periodic infusion of genetically engineered lymphocytes because : Answer: 1. Genetically engineered lymphocytes are not immortal cells. Key idea: Lymphocytes are short-lived, so the gene-corrected cells mus... 1
- The Adenosine deaminase deficiency results into : Answer: 1. Dysfunction of Immune system Adenosine deaminase (ADA) deficiency damages lymphocytes and causes severe combined immunodeficiency... 1
- With regard to insulin choose correct options.(a) C-peptide is not present in mature insulin.(b) The insulin produced by rDNA technology has C-peptide.(c) The pro-insulin has C-peptide.(d) A-peptide and B-peptide of insulin are interconnected by disu… Answer: 3. (a), (c) and (d) only Pro-insulin has chains A, B and a connecting C-peptide. During maturation C-peptide is removed, leaving A a... 1
- When gene targetting involving gene amplification is attempted in an individual’s tissue to treat disease, it is known as : Answer: 2. Gene therapy Treating a disease by correcting a defective gene, by delivering a normal gene into a person's cells and tissues, is... 1
- Match the following columns and select the correct option.Column - I | Column - II(a) Bt cotton | (i) Gene therapy(b) Adenosine deaminase deficiency | (ii) Cellular defence(c) RNAi | (iii) Detection of HIV infection(d) PCR | (iv) Bacillus thuringiens… Answer: 1. (a) (iv), (b) (i), (c) (ii), (d) (iii) Bt cotton uses a gene from Bacillus thuringiensis for pest resistance: (a) - (iv). Adenosi... 1
- Which of the following statements is not correct ? Answer: 3. The functional insulin has A and B chains linked together by hydrogen bonds. Key idea: Mature insulin chains A and B are joined b... 1
- Which kind of therapy was given in 1990 to a four-year-old girl with adenosine deaminase (ADA) deficiency? Answer: 4. Gene therapy In 1990 a four-year-old girl with ADA deficiency received the first clinical gene therapy: lymphocytes were taken, a... 1