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Tools: restriction enzymes, vectors, host

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Every question from past board papers on Tools: restriction enzymes, vectors, host, newest paper first. Open any question for its full worked answer.

NEET UG 2025 question paper (official, booklet 45)

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  1. In the above represented plasmid an alien piece of DNA is inserted at EcoRI site. Which of the following strategies will be chosen to select the recombinant colonies?

    Plasmid map with BamHI, Tet R, Ori, EcoRI, beta-Galactosidase, amp

    4 marks · See worked answer →

  2. Which of the following enzyme(s) are NOT essential for gene cloning?

    A. Restriction enzymes

    B. DNA ligase

    C. DNA mutase

    D. DNA recombinase

    E. DNA polymerase

    Choose the correct answer from the options given below :

    4 marks · See worked answer →

  3. The blue and white selectable markers have been developed which differentiate recombinant colonies from non-recombinant colonies on the basis of their ability to produce colour in presence of a chromogenic substrate.

    Given below are two statements about this method:

    Statement I : The blue coloured colonies have DNA insert in the plasmid and they are identified as recombinant colonies.

    Statement II : The colonies without blue colour have DNA insert in the plasmid and are identified as recombinant colonies.

    In the light of the above statements, choose the most appropriate answer from the options given below :

    4 marks · See worked answer →

NEET UG 2024 question paper (official, booklet S6)

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  1. Hind II always cuts DNA molecules at a particular point called recognition sequence and it consists of:

    4 marks · See worked answer →

  2. The "Ti plasmid" of Agrobacterium tumefaciens stands for

    4 marks · See worked answer →

  3. The following diagram showing restriction sites in E.coli cloning vector pBR322. Find the role of 'X' and 'Y' genes :

    pBR322 plasmid map with restriction sites and genes X and Y

    4 marks · See worked answer →

NEET UG 2023 question paper (official, booklet G6)

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  1. Which of the following is not a cloning vector?

    4 marks · See worked answer →

NEET UG 2022 question paper (official, booklet R6)

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  1. In the following palindromic base sequences of DNA, which one can be cut easily by particular restriction enzyme ?

    4 marks · See worked answer →

  2. Transposons can be used during which one of the following ?

    4 marks · See worked answer →

  3. Given below are two statements :

    Statement I :

    Restriction endonucleases recognise specific sequence to cut DNA known as palindromic nucleotide sequence.

    Statement II :

    Restriction endonucleases cut the DNA strand a little away from the centre of the palindromic site.

    In the light of the above statements, choose the most appropriate answer from the options given below :

    4 marks · See worked answer →

  4. Which of the following is not a desirable feature of a cloning vector ?

    4 marks · See worked answer →

NEET UG 2021 question paper (official, booklet M4)

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  1. Plasmid pBR322 has PstI restriction enzyme site within gene ampR that confers ampicillin resistance. If this enzyme is used for inserting a gene for β-galactoside production and the recombinant plasmid is inserted in an E.coli strain

    4 marks · See worked answer →

  2. A specific recognition sequence identified by endonucleases to make cuts at specific positions within the DNA is :

    4 marks · See worked answer →

NEET UG 2020 question paper (official, booklet E1)

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  1. Choose the correct pair from the following :

    4 marks · See worked answer →

  2. The specific palindromic sequence which is recognized by EcoRI is :

    4 marks · See worked answer →

  3. Identify the wrong statement with regard to Restriction Enzymes.

    4 marks · See worked answer →

  4. Match the organism with its use in biotechnology.

    (a) Bacillus thuringiensis (i) Cloning vector

    (b) Thermus aquaticus (ii) Construction of first rDNA molecule

    (c) Agrobacterium tumefaciens (iii) DNA polymerase

    (d) Salmonella typhimurium (iv) Cry proteins

    Select the correct option from the following :

    4 marks · See worked answer →

  5. The sequence that controls the copy number of the linked DNA in the vector, is termed :

    4 marks · See worked answer →

NEET UG 2019 question paper (official, booklet P1)

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  1. Following statements describe the characteristics of the enzyme Restriction Endonuclease. Identify the incorrect statement.

    4 marks · See worked answer →

NEET UG 2018 question paper (official, booklet EE)

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  1. Which of the following is commonly used as a vector for introducing a DNA fragment in human lymphocytes ?

    4 marks · See worked answer →

NEET UG 2017 question paper (official, booklet B)

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  1. A gene whose expression helps to identify transformed cell is known as :

    4 marks · See worked answer →

NEET UG 2016 Phase 1 question paper (official, booklet A)

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  1. Which of the following is not a feature of the plasmids ?

    4 marks · See worked answer →

  2. Which of the following is a restriction endonuclease ?

    4 marks · See worked answer →

NEET UG 2016 Phase 2 question paper (official, booklet ZZ)

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  1. Which of the following restriction enzymes produces blunt ends?

    4 marks · See worked answer →