Tools: restriction enzymes, vectors, host

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Which of the following enzyme(s) are NOT essential for gene cloning?A. Restriction enzymesB. DNA ligaseC. DNA mutaseD. DNA recombinaseE. DNA polymeraseChoose the correct answer from the options given below : Answer: 1. C and D only Key idea: Gene cloning needs enzymes to cut (restriction enzymes), join (DNA ligase), and make copies (DNA polymeras... Tools: restriction enzymes, vectors, host 1 answer
The blue and white selectable markers have been developed which differentiate recombinant colonies from non-recombinant colonies on the basis of their ability to produce colour in presence of a chromogenic substrate.Given below are two statements abo… Answer: 4. Statement I is incorrect but Statement II is correct Key idea: Insertional inactivation of the lacZ gene (β-galactosidase). In a... Tools: restriction enzymes, vectors, host 1 answer
In the above represented plasmid an alien piece of DNA is inserted at EcoRI site. Which of the following strategies will be chosen to select the recombinant colonies? Answer: 3. White color colonies will be selected. Key idea: The figure shows the EcoRI site inside the gene for β -galactosidase. Inserting... Tools: restriction enzymes, vectors, host 1 answer
The "Ti plasmid" of Agrobacterium tumefaciens stands for Answer: 4. Tumor inducing plasmid The Ti plasmid of Agrobacterium tumefaciens is the tumour-inducing plasmid. Part of it (T-DNA) enters plan... Tools: restriction enzymes, vectors, host 1 answer
The following diagram showing restriction sites in E.coli cloning vector pBR322. Find the role of 'X' and 'Y' genes : Answer: 3. The gene 'X' is responsible for controlling the copy number of the linked DNA and 'Y' for protein involved in the replication of... Tools: restriction enzymes, vectors, host 1 answer
Hind II always cuts DNA molecules at a particular point called recognition sequence and it consists of: Answer: 3. 6 bp Key idea: restriction enzymes such as Hind II recognise a specific palindromic sequence of 6 base pairs. Hind II recognises... Tools: restriction enzymes, vectors, host 1 answer
Which of the following is not a cloning vector? Answer: 1. Probe Key idea: A cloning vector carries foreign DNA into a host and replicates there. BAC (bacterial artificial chromosome), YAC... Tools: restriction enzymes, vectors, host 1 answer
Which of the following is not a desirable feature of a cloning vector ? Answer: 1. Presence of two or more recognition sites Key idea: A good cloning vector should have a single recognition site for the restricti... Tools: restriction enzymes, vectors, host 1 answer
In the following palindromic base sequences of DNA, which one can be cut easily by particular restriction enzyme ? Answer: 3. 5'GAATTC3'; 3'CTTAAG5' A palindromic site reads the same 5' to 3' on both strands. 5'GAATTC3' with complementary 3'CTTAAG5' is a... Tools: restriction enzymes, vectors, host 1 answer
Transposons can be used during which one of the following ? Answer: 3. Gene silencing As per the official key, the answer is gene silencing: transposons (jumping genes) can insert in or near a gene an... Tools: restriction enzymes, vectors, host 1 answer
Given below are two statements :Statement I :Restriction endonucleases recognise specific sequence to cut DNA known as palindromic nucleotide sequence.Statement II :Restriction endonucleases cut the DNA strand a little away from the centre of the pal… Answer: 2. Both Statement I and Statement II are correct Key idea: Restriction enzymes recognise palindromic sequences and cut each strand s... Tools: restriction enzymes, vectors, host 1 answer
Plasmid pBR322 has PstI restriction enzyme site within gene ampR that confers ampicillin resistance. If this enzyme is used for inserting a gene for β-galactoside production and the recombinant plasmid is inserted in an E.coli strain Answer: 1. it will not be able to confer ampicillin resistance to the host cell. The PstI site lies inside the ampR gene. Inserting the fore... Tools: restriction enzymes, vectors, host 1 answer
A specific recognition sequence identified by endonucleases to make cuts at specific positions within the DNA is : Answer: 3. Palindromic Nucleotide sequences Restriction endonucleases recognise specific palindromic nucleotide sequences in DNA and cut at... Tools: restriction enzymes, vectors, host 1 answer
The sequence that controls the copy number of the linked DNA in the vector, is termed : Answer: 2. Ori site The origin of replication (ori) is the sequence from where replication starts, and it controls the copy number of the li... Tools: restriction enzymes, vectors, host 1 answer
The specific palindromic sequence which is recognized by EcoRI is : Answer: 1. 5' - GAATTC - 3'3' - CTTAAG - 5' Key idea: EcoRI recognises 5'-GAATTC-3' and cuts between G and A. A palindrome reads the same on... Tools: restriction enzymes, vectors, host 1 answer
Identify the wrong statement with regard to Restriction Enzymes. Answer: 4. Sticky ends can be joined by using DNA ligases. Key idea: Judge each statement against the NCERT chapter on restriction enzymes.... Tools: restriction enzymes, vectors, host 1 answer
Match the organism with its use in biotechnology.(a) Bacillus thuringiensis (i) Cloning vector(b) Thermus aquaticus (ii) Construction of first rDNA molecule(c) Agrobacterium tumefaciens (iii) DNA polymerase(d) Salmonella typhimurium (iv) Cry proteins… Answer: 2. (a) (iv), (b) (iii), (c) (i), (d) (ii) Key idea: Match each organism with its biotechnology use. Bacillus thuringiensis: Cry prot... Tools: restriction enzymes, vectors, host 1 answer
Choose the correct pair from the following : Answer: 1. Ligases - Join the two DNA molecules Key idea: Ligases join DNA fragments. DNA ligase seals the sugar-phosphate backbone between... Tools: restriction enzymes, vectors, host 1 answer
Following statements describe the characteristics of the enzyme Restriction Endonuclease. Identify the incorrect statement. Answer: 2. The enzyme binds DNA at specific sites and cuts only one of the two strands. Key idea: Restriction endonucleases cut both strands... Tools: restriction enzymes, vectors, host 1 answer
Which of the following is commonly used as a vector for introducing a DNA fragment in human lymphocytes ? Answer: 1. Retrovirus To put a gene into animal cells such as human lymphocytes, disarmed retroviruses are used as vectors. Retroviruses inf... Tools: restriction enzymes, vectors, host 1 answer
A gene whose expression helps to identify transformed cell is known as : Answer: 4. Selectable marker A selectable marker is a gene whose expression lets us identify and select transformed cells, for example an an... Tools: restriction enzymes, vectors, host 1 answer
Which of the following restriction enzymes produces blunt ends? Answer: 1. *Eco* RV EcoRV cuts in the middle of its recognition site (GAT^ATC) and gives blunt ends. XhoI, HindIII and SalI cut with a stagg... Tools: restriction enzymes, vectors, host 1 answer
Which of the following is a restriction endonuclease ? Answer: 1. Hind II Hind II is a restriction endonuclease, an enzyme from bacteria that cuts DNA at specific recognition sequences. Protease... Tools: restriction enzymes, vectors, host 1 answer
Which of the following is not a feature of the plasmids ? Answer: 4. Single - stranded Key idea: Plasmids are double-stranded, circular, extra-chromosomal DNA. They replicate independently of the ch... Tools: restriction enzymes, vectors, host 1 answer