Biotechnology and Its Applications

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In the following palindromic base sequences of DNA, which one can be cut easily by particular restriction enzyme ? Answer: 3. 5'GAATTC3'; 3'CTTAAG5' A palindromic site reads the same 5' to 3' on both strands. 5'GAATTC3' with complementary 3'CTTAAG5' is a... Tools: restriction enzymes, vectors, host 1 answer
Transposons can be used during which one of the following ? Answer: 3. Gene silencing As per the official key, the answer is gene silencing: transposons (jumping genes) can insert in or near a gene an... Tools: restriction enzymes, vectors, host 1 answer
Given below are two statements :Statement I :Restriction endonucleases recognise specific sequence to cut DNA known as palindromic nucleotide sequence.Statement II :Restriction endonucleases cut the DNA strand a little away from the centre of the pal… Answer: 2. Both Statement I and Statement II are correct Key idea: Restriction enzymes recognise palindromic sequences and cut each strand s... Tools: restriction enzymes, vectors, host 1 answer
Which one of the following statement is not true regarding gel electrophoresis technique ? Answer: 4. The presence of chromogenic substrate gives blue coloured DNA bands on the gel. In gel electrophoresis, DNA fragments are stained... Processes of recombinant DNA technology 1 answer
Given below are two statements : one is labelled as Assertion (A) and the other is labelled as Reason (R).Assertion (A) :Polymerase chain reaction is used in DNA amplificationReason (R) :The ampicillin resistant gene is used as a selectable marker to… Answer: 3. Both (A) and (R) are correct but (R) is not the correct explanation of (A) Check each statement and then the link between them. A... Processes of recombinant DNA technology 1 answer
The Adenosine deaminase deficiency results into : Answer: 1. Dysfunction of Immune system Adenosine deaminase (ADA) deficiency damages lymphocytes and causes severe combined immunodeficiency... Applications in health: human insulin, vaccines, gene therapy 1 answer
With regard to insulin choose correct options.(a) C-peptide is not present in mature insulin.(b) The insulin produced by rDNA technology has C-peptide.(c) The pro-insulin has C-peptide.(d) A-peptide and B-peptide of insulin are interconnected by disu… Answer: 3. (a), (c) and (d) only Pro-insulin has chains A, B and a connecting C-peptide. During maturation C-peptide is removed, leaving A a... Applications in health: human insulin, vaccines, gene therapy 1 answer
For effective treatment of the disease, early diagnosis and understanding its pathophysiology is very important. Which of the following molecular diagnostic techniques is very useful for early detection ? Answer: 3. ELISA Technique ELISA detects antigens or antibodies and can reveal infection (e.g. HIV) before symptoms appear, so it is the tec... Processes of recombinant DNA technology 1 answer
During the process of gene amplification using PCR, if very high temperature is not maintained in the beginning, then which of the following steps of PCR will be affected first ? Answer: 3. Denaturation PCR starts with denaturation at about 94°C, which separates the double-stranded DNA into single strands. Without thi... Processes of recombinant DNA technology 1 answer
Now a days it is possible to detect the mutated gene causing cancer by allowing radioactive probe to hybridise its complimentary DNA in a clone of cells, followed by its detection using autoradiography because : Answer: 3. mutated gene does not appear on a photographic film as the probe has no complimentarity with it. The radioactive probe is a singl... Processes of recombinant DNA technology 1 answer
Plasmid pBR322 has PstI restriction enzyme site within gene ampR that confers ampicillin resistance. If this enzyme is used for inserting a gene for β-galactoside production and the recombinant plasmid is inserted in an E.coli strain Answer: 1. it will not be able to confer ampicillin resistance to the host cell. The PstI site lies inside the ampR gene. Inserting the fore... Tools: restriction enzymes, vectors, host 1 answer
A specific recognition sequence identified by endonucleases to make cuts at specific positions within the DNA is : Answer: 3. Palindromic Nucleotide sequences Restriction endonucleases recognise specific palindromic nucleotide sequences in DNA and cut at... Tools: restriction enzymes, vectors, host 1 answer
When gene targetting involving gene amplification is attempted in an individual’s tissue to treat disease, it is known as : Answer: 2. Gene therapy Treating a disease by correcting a defective gene, by delivering a normal gene into a person's cells and tissues, is... Applications in health: human insulin, vaccines, gene therapy 1 answer
Which of the following is not an application of PCR (Polymerase Chain Reaction) ? Answer: 3. Purification of isolated protein PCR amplifies a chosen DNA segment from small amounts of DNA. It is used for gene amplification,... Processes of recombinant DNA technology 1 answer
During the purification process for recombinant DNA technology, addition of chilled ethanol precipitates out : Answer: 2. DNA DNA is insoluble in ethanol. When chilled ethanol is added to the purified lysate, DNA precipitates and appears as fine threa... Processes of recombinant DNA technology 1 answer
Which of the following is a correct sequence of steps in a PCR (Polymerase Chain Reaction) ? Answer: 1. Denaturation, Annealing, Extension Each PCR cycle has three steps in order: denaturation (double strand separated at about 94 °C)... Processes of recombinant DNA technology 1 answer
The sequence that controls the copy number of the linked DNA in the vector, is termed : Answer: 2. Ori site The origin of replication (ori) is the sequence from where replication starts, and it controls the copy number of the li... Tools: restriction enzymes, vectors, host 1 answer
Match the following columns and select the correct option.Column - I | Column - II(a) Bt cotton | (i) Gene therapy(b) Adenosine deaminase deficiency | (ii) Cellular defence(c) RNAi | (iii) Detection of HIV infection(d) PCR | (iv) Bacillus thuringiens… Answer: 1. (a) (iv), (b) (i), (c) (ii), (d) (iii) Bt cotton uses a gene from Bacillus thuringiensis for pest resistance: (a) - (iv). Adenosi... Applications in health: human insulin, vaccines, gene therapy 1 answer
Bt cotton variety that was developed by the introduction of toxin gene of Bacillus thuringiensis (Bt) is resistant to : Answer: 1. Insect pests Key idea: The Bt toxin (Cry protein) kills certain insects. Bt cotton is resistant to insect pests such as cotton bo... Applications in agriculture: GMOs, Bt crops 1 answer
Which of the following statements is not correct ? Answer: 3. The functional insulin has A and B chains linked together by hydrogen bonds. Key idea: Mature insulin chains A and B are joined b... Applications in health: human insulin, vaccines, gene therapy 1 answer
The specific palindromic sequence which is recognized by EcoRI is : Answer: 1. 5' - GAATTC - 3'3' - CTTAAG - 5' Key idea: EcoRI recognises 5'-GAATTC-3' and cuts between G and A. A palindrome reads the same on... Tools: restriction enzymes, vectors, host 1 answer
Identify the wrong statement with regard to Restriction Enzymes. Answer: 4. Sticky ends can be joined by using DNA ligases. Key idea: Judge each statement against the NCERT chapter on restriction enzymes.... Tools: restriction enzymes, vectors, host 1 answer
Match the organism with its use in biotechnology.(a) Bacillus thuringiensis (i) Cloning vector(b) Thermus aquaticus (ii) Construction of first rDNA molecule(c) Agrobacterium tumefaciens (iii) DNA polymerase(d) Salmonella typhimurium (iv) Cry proteins… Answer: 2. (a) (iv), (b) (iii), (c) (i), (d) (ii) Key idea: Match each organism with its biotechnology use. Bacillus thuringiensis: Cry prot... Tools: restriction enzymes, vectors, host 1 answer
In gel electrophoresis, separated DNA fragments can be visualized with the help of : Answer: 2. Ethidium bromide in UV radiation Key idea: DNA in agarose gels is stained with ethidium bromide and viewed under UV light. Ethidi... Processes of recombinant DNA technology 1 answer

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